Dialytrode technology and local profiles of amino acids in the awake cat brain

Methods to investigate the in vivo effects and release of neuroactive substances include cortical cups, push-pull cannulae, chemitrodes, and dialytrodes. Critical evaluation of these procedures is necessary in order to interpret related results and to select the most suitable devices for further studies. Recent improvements in the dialytrode include structural modifications and the use of a small, permeable membrane constructed of thin polyester. The dialytrode system and its diffusion rates have been characterized with in vitro studies. In vivo long-term experiments in awake cats have been conducted to test injection rates, diffusion of [14C]urea, temporal variability, pressure factors, and other experimental variables. Using dialytrodes we have measured the normal profile of amino acids present in different cerebral structures and their possible correlations.