Direct determination of calcium and magnesium in serum using flow-injection analysis and atomic absorption...

The use of flow-injection analysis for the direct determination of calcium and magnesium in blood serum and plasma is described. An inexpensive rotary valve is used to inject the serum sample (4 microliters) into a flowing non-segmented stream of reagent which carries the sample slug through a long narrow-bore coil--where it gradually disperses--and into the nebuliser of an atomic absorption spectrometer. This on-stream sample dilution removes the need for predilution of the sample. The resulting absorbance signals are recorded as peaks less than 40 seconds after sample injection. Analytical recoveries and precision are good for both elements and the results by flow-injection analysis compare well with established routine methods.