Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human platelet fibrinogen gamma chain structure.

M W Mosesson, G A Homandberg, D L Amrani

    Blood
    |May 1, 1984
    PubMed
    Summary

    Platelet fibrinogen lacks gamma'-chains, unlike plasma fibrinogen produced by the liver. This suggests distinct gene regulation in megakaryocytes, impacting fibrin structure and function.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evidence for covalent linkage between some plasma α2-antiplasmin molecules and Aα chains of circulating fibrinogen.

    Journal of thrombosis and haemostasis : JTH·2013
    Same author

    Mechanical impact induces cartilage degradation via mitogen activated protein kinases.

    Osteoarthritis and cartilage·2010
    Same author

    Short by one mechanism: a rebuttal.

    Journal of thrombosis and haemostasis : JTH·2010
    Same author

    Human fibrinogen heterogeneities: determination of the major Aalpha chain derivatives in blood.

    Thrombosis research·2010
    Same author

    Fibronectin fragments mediate matrix metalloproteinase upregulation and cartilage damage through proline rich tyrosine kinase 2, c-src, NF-kappaB and protein kinase Cdelta.

    Osteoarthritis and cartilage·2009
    Same author

    Telopeptides of type II collagen upregulate proteinases and damage cartilage but are less effective than highly active fibronectin fragments.

    Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2009

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Hematology

    Background:

    • Human plasma fibrinogen, synthesized in the liver, contains two gamma-chain types (gamma A, gamma"), differing in COOH-terminal sequences.
    • Crosslinking of plasma fibrin by factor XIIIa and Ca2+ yields three gamma-dimer types: gamma A-gamma A, gamma A-gamma", and gamma"-gamma".

    Purpose of the Study:

    • To investigate the presence and extent of gamma ahydrogen-chains in platelet alpha-granule fibrinogen.
    • To compare the structure of platelet fibrinogen with hepatic fibrinogen.

    Main Methods:

    • Electrophoretic analysis (Laemmli system) of reduced platelet fibrin clots.
    • Western blotting using anti-gamma-chain antibodies on platelet fibrinogen lysates and fibrin.
    • Analysis of fibrinogen from thrombin-stimulated washed platelets and Triton X-100/urea lysates.

    Main Results:

    • Electrophoresis revealed a single gamma A-gamma A dimer band in crosslinked platelet fibrin.
    • Western blotting confirmed the exclusive presence of gamma A-chains in platelet fibrinogen.
    • No detectable gamma ahydrogen-chains (<1%) were found in platelet fibrinogen.

    Conclusions:

    • Platelet fibrinogen is structurally distinct from hepatic fibrinogen due to the absence of gamma ahydrogen-chains.
    • This difference likely arises from unique processing or regulatory mechanisms of the fibrinogen gamma-chain gene in megakaryocytes.

    Related Experiment Videos