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Updated: Jul 16, 2026

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
Published on: March 30, 2010
Chemical characterization of cyanogen bromide fragments from the beta-chain of human complement factor C3
Abstract:
The isolated beta-chain of human complement factor C3 (C3 beta) was fragmented by cyanogen bromide. Nine fragments were defined by gel filtration and high-pressure liquid chromatography, and characterized with respect to their Mr, amino acid composition and N-terminal amino acid sequence. Approx. 30% of the primary structure of C3 beta was determined. Alignment of the 3 N-terminal fragments allowed determination of 61 of the amino terminal residues of C3 beta. This region demonstrated 40% homology with the sequence in the N-terminal segment of the alpha-chain of the cobra venom factor.
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