Direct effect of triiodothyronine on glucose utilization in adipocytes from obese and nonobese Zucker rats

The direct effects of L-T3 (triiodothyronine) on glucose utilization, 2-deoxyglucose uptake and O2 consumption were evaluated in vitro in isolated adipocytes from 6-week-old obese and nonobese Zucker rats. Adipocytes treated for 30 min with L-T3 had a 20-25% decrease in glucose conversion to CO2, fatty acids and glyceride-glycerol and uptake of 2-deoxyglucose. Incubation of adipocytes with either D-T3 or rT3 (reverse T3) did not decrease utilization of glucose. T3 also decreased the insulin-stimulated fatty acid synthesis from glucose in both obese and nonobese adipocytes. Adipocyte O2 consumption was decreased by T3 in media that contained glucose but not in media that contained either acetate or no exogenous substrate. These data indicate that T3 has a direct, immediate and specific action on adipocyte glucose utilization and may act to modulate adipose lipogenesis and lipolysis.