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Related Experiment Videos

Urinary immunoreactive gastrin in normal subjects.

B Du, J Zhang, J Eng

    Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
    |March 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

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    Researchers developed a method to measure gastrin in urine using C18 Sep-Pak cartridges and radioimmunoassay. This technique accurately quantifies gastrin levels, aiding in understanding its excretion and clearance in normal subjects.

    Area of Science:

    • Endocrinology
    • Nephrology
    • Analytical Chemistry

    Background:

    • Gastrin, a peptide hormone, plays a crucial role in digestion and gastrointestinal function.
    • Accurate measurement of gastrin is essential for understanding its physiological roles and potential disease associations.
    • Existing methods for gastrin measurement may have limitations in sensitivity or specificity, particularly in complex biological matrices like urine.

    Purpose of the Study:

    • To develop and validate a reliable method for concentrating and measuring gastrin in human urine.
    • To characterize the forms of gastrin excreted in urine.
    • To assess the renal clearance and urinary excretion of gastrin in normal individuals under different physiological conditions (fasted vs. fed).

    Main Methods:

    • Urine samples (10-50 ml) were processed using octadecylsilyl (ODS) silica columns (C18 Sep-Pak cartridge) for gastrin concentration.

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  • Concentrated gastrin was quantified using radioimmunoassay.
  • Fractionation of urinary gastrin was performed using Sephadex G50 gel filtration.
  • Renal clearance and urinary gastrin output were measured in normal subjects under fasted and fed states.

    • Main Results:

    • Octadecylsilyl (ODS) silica columns achieved >90% recovery for gastrin concentration from urine.
    • Radioimmunoassay, following C18 Sep-Pak purification, confirmed the presence of both 17 and 34 amino acid gastrin forms in urine.
    • Renal clearance of gastrin averaged 0.16 +/- 0.05 ml/min and did not differ significantly between fasted and fed states.
    • Urinary gastrin excretion was low in the fasting state (<0.005 pmol/hr/kg) and increased post-feeding, averaging 8.5 +/- 1.5 pmol/24 hr.

    Conclusions:

    • A robust method utilizing C18 Sep-Pak cartridges and radioimmunoassay allows for efficient concentration and measurement of urinary gastrin.
    • Urinary gastrin excretion patterns reflect feeding status, suggesting a role for renal excretion in gastrin homeostasis.
    • Renal gastrin clearance and 24-hour urinary output measurements can provide insights into average plasma gastrin levels in individuals without renal disease.
    • The described methodology is potentially adaptable for the analysis of other peptide hormones in biological fluids.