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Simultaneous measurement of adherence and chemiluminescence by polymorphonuclear leukocytes
Abstract:
Oxygen radical release from adhering polymorphonuclear leukocytes (PMN) has been implicated as an important feature of many vascular diseases. We developed a technique by which adherence and production of O2 radicals by PMN can be measured simultaneously. The technique combines the conventional nylon fiber assay for measuring adherence of PMN with concurrent scintillation counter measurement of chemiluminescence (CL) to assess O2 radical production by PMN. We found that adherence of PMN to nylon fiber is associated with increases in CL. Moreover, increases in CL appear to be dependent on generation of O2 radicals from PMN since they are not seen with PMN from a patient with chronic granulomatous disease (CGD) or in the presence of O2 radical scavengers, superoxide dismutase, or catalase. Furthermore, agents which increase the adherence of PMN to nylon fiber are associated with increases in CL. Use of this approach may facilitate simultaneous evaluation of adherence and O2 radical generation by PMN.
Insights
This study introduces a new method to measure polymorphonuclear leukocyte (PMN) adherence and oxygen radical release simultaneously. The technique links PMN adherence to nylon fibers with chemiluminescence, aiding vascular disease research.
Area of Science:
- Biomedical Engineering
- Immunology
- Vascular Biology
Background:
- Oxygen radical release from adhering polymorphonuclear leukocytes (PMN) is a key factor in vascular diseases.
- Simultaneous measurement of PMN adherence and oxygen radical production is crucial for understanding these conditions.
Purpose of the Study:
- To develop and validate a novel technique for simultaneously measuring PMN adherence and O2 radical production.
- To investigate the relationship between PMN adherence and O2 radical generation.
Main Methods:
- Combined a conventional nylon fiber adherence assay with scintillation counter measurement of chemiluminescence (CL).
- Assessed O2 radical production by measuring CL, a byproduct of oxygen radical activity.
Main Results:
- PMN adherence to nylon fibers correlated with increased CL.
- Increased CL was dependent on O2 radical generation, as evidenced by studies with chronic granulomatous disease (CGD) PMN and radical scavengers.
- Agents enhancing PMN adherence also increased CL.
Conclusions:
- The developed technique allows for simultaneous evaluation of PMN adherence and O2 radical generation.
- This method can facilitate further research into the role of PMN in vascular diseases.