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Susceptibility and resistance of inbred mice to Giardia muris
Abstract:
The susceptibility and resistance of inbred mice to Giardia muris was studied during the acute and elimination phases of infection. The infection in susceptible A/J and C3H/He mice was characterized by a short latent period, high cyst output during the acute phase of infection, and prolonged periods of cyst release. In contrast, resistant B10.A and DBA/2 mice had a longer latent period, a lower cyst output during the acute phase, and relatively rapid resolution of infection. The trait of susceptibility and resistance during both acute and elimination phases of the infection was found to be under complex multigenic control as determined by examination of the response of F1 hybrid mice and backcross analyses. The genes controlling this trait did not appear to be linked to the H-2 locus. In addition, the control of the response of mice to G. muris during the acute phase of infection was probably mediated by mechanisms independent from those controlling the response during the elimination phase of infection.
Insights
Genetic factors influence mouse susceptibility to Giardia muris infection. Resistant mice clear the parasite faster than susceptible strains, indicating complex multigenic control independent of the H-2 locus.
Area of Science:
- Immunology
- Genetics
- Infectious Diseases
Background:
- Giardia muris is an intestinal protozoan parasite.
- Mouse models are crucial for understanding host-parasite interactions.
Purpose of the Study:
- To investigate the genetic basis of susceptibility and resistance to Giardia muris in mice.
- To determine the phases of infection influenced by host genetics.
Main Methods:
- Comparative study of inbred mouse strains (A/J, C3H/He, B10.A, DBA/2).
- Analysis of F1 hybrid mice and backcrosses to determine genetic control.
- Assessment of infection parameters: latent period, cyst output, and resolution time.
Main Results:
- Susceptible mice (A/J, C3H/He) exhibited shorter latency, higher cyst output, and prolonged shedding.
- Resistant mice (B10.A, DBA/2) showed longer latency, lower cyst output, and faster resolution.
- Genetic analysis indicated complex multigenic control, not linked to the H-2 locus.
- Acute and elimination phases of infection are likely controlled by independent genetic mechanisms.
Conclusions:
- Host genetic background significantly dictates the course of Giardia muris infection in mice.
- Multiple genes, independent of the H-2 complex, regulate both acute and resolution phases.
- Understanding these genetic factors can inform strategies for controlling Giardia infections.