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Disk diffusion susceptibility testing of ticarcillin plus clavulanic acid.

P C Fuchs, R N Jones, A L Barry

Journal of Clinical Microbiology

|April 1, 1984

Summary

A new disk diffusion test using ticarcillin and clavulanic acid shows promise for bacterial susceptibility testing. The 75-10 microgram disk is recommended for evaluating ticarcillin-clavulanic acid effectiveness.

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Area of Science:

Microbiology
Clinical Laboratory Science
Antimicrobial Susceptibility Testing

Background:

Ticarcillin is an antibiotic often combined with clavulanic acid to overcome beta-lactamase resistance.
Accurate susceptibility testing is crucial for guiding antibiotic therapy.

Purpose of the Study:

To evaluate the disk diffusion method for testing susceptibility to ticarcillin plus clavulanic acid.
To compare disk diffusion results with reference broth microdilution methods.

Main Methods:

Disk diffusion susceptibility testing was performed on 489 clinical isolates.
Four different disk concentrations of ticarcillin (75 µg) with clavulanic acid (2.5, 5, 10, 15 µg) were tested.
Results were compared against reference broth microdilution susceptibilities.

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Main Results:

The disk containing 75 µg of ticarcillin and 10 µg of clavulanic acid demonstrated good performance.
The established interpretive criteria for ticarcillin appeared suitable for the combination drug.

Conclusions:

The 75-10 µg disk diffusion test is a recommended method for ticarcillin-clavulanic acid susceptibility.
Further validation is needed due to a limited number of resistant isolates in this study.