Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interactions between renal brush border membranes and polyamines.

B B Kirschbaum

    The Journal of Pharmacology and Experimental Therapeutics
    |May 1, 1984
    PubMed
    Summary

    Aminoglycosides like gentamicin can alter kidney cell membranes by binding to anionic phospholipids. This interaction, even at low doses, affects membrane structure and function, potentially explaining kidney damage.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Hyperaluminaemia associated with oral citrate and aluminium hydroxide.

    Human toxicology·1989
    Same author

    Acute aluminum toxicity associated with oral citrate and aluminum-containing antacids.

    The American journal of the medical sciences·1989
    Same author

    Analysis of reciprocal creatinine plots in renal failure.

    The American journal of the medical sciences·1986
    Same author

    Further discussion of a urine specific gravity test strip.

    Clinical chemistry·1984
    Same author

    Hypocalciuric hyperparathyroidism with chronic renal failure.

    Southern medical journal·1983
    Same author

    Evaluation of a colorimetric reagent strip assay for urine specific gravity.

    American journal of clinical pathology·1983

    Area of Science:

    • Nephrology
    • Biochemistry
    • Pharmacology

    Background:

    • Aminoglycosides accumulate in the renal cortex, causing tubular damage.
    • The precise mechanism of aminoglycoside-induced nephrotoxicity at the membrane level requires further elucidation.

    Purpose of the Study:

    • To investigate the interaction characteristics between aminoglycosides and kidney brush border membranes.
    • To understand how aminoglycosides affect membrane structure and function.

    Main Methods:

    • Assay of isolated kidney brush border membrane aggregation induced by gentamicin and netilmicin.
    • Fluorescence analysis using 8-anilino-1-naphthalene sulfonic acid to assess membrane changes.
    • Competition assays with other drugs and spermine to identify interaction sites.

    Main Results:

    • Aminoglycosides induced aggregation of isolated kidney brush border membranes.
    • Competition observed between aminoglycosides and Ca++ or drugs causing phospholipidosis.
    • Gentamicin, even at sub-therapeutic levels, enhanced 8-anilino-1-naphthalene sulfonic acid fluorescence, indicating membrane perturbation.
    • Effects of gentamicin and Ca++ on fluorescence were additive.

    Conclusions:

    • Aminoglycosides directly interact with and modify kidney brush border membrane structure.
    • These interactions involve anionic phospholipids and may be influenced by native polyamines.
    • Subtoxic levels of aminoglycosides can alter membrane characteristics, warranting further investigation into physiological consequences.

    Related Experiment Videos