Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intermolecular ligand substitution reactions.

W T Jenkins

    Progress in Clinical and Biological Research
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Enzyme ligand complex formation is best understood as ion exchange or substitution reactions. Using surrogate ligands and constant ratios simplifies kinetic analysis for better mechanistic insights.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Noninvasive imaging of tumor hypoxia in rats using the 2-nitroimidazole 18F-EF5.

    European journal of nuclear medicine and molecular imaging·2003
    Same author

    Effects of hyperglycemia on oxygenation, radiosensitivity and bioenergetic status of subcutaneous RIF-1 tumors.

    International journal of oncology·2002
    Same author

    Doppler ultrasound imaging detects changes in tumor perfusion during antivascular therapy associated with vascular anatomic alterations.

    Cancer research·2001
    Same author

    Hypoxia in human intraperitoneal and extremity sarcomas.

    International journal of radiation oncology, biology, physics·2001
    Same author

    Detection of hypoxia in human squamous cell carcinoma by EF5 binding.

    Cancer research·2000
    Same author

    Hypoxia and necrosis in rat 9L glioma and Morris 7777 hepatoma tumors: comparative measurements using EF5 binding and the Eppendorf needle electrode.

    International journal of radiation oncology, biology, physics·2000

    Area of Science:

    • Biochemistry
    • Chemical Kinetics

    Background:

    • Enzyme-ligand interactions are fundamental to biochemical processes.
    • Understanding complex formation and dissociation mechanisms is crucial for enzyme function studies.

    Purpose of the Study:

    • To propose a novel perspective on enzyme-ligand complex formation.
    • To demonstrate the utility of surrogate ligands in mechanistic studies.
    • To introduce a simplified kinetic analysis method.

    Main Methods:

    • Conceptualizing enzyme-ligand complex formation as intermolecular ion exchange or ligand substitution.
    • Employing surrogate ligands (e.g., buffer ions, inhibitors) to probe reaction mechanisms.
    • Maintaining constant substrate/cosubstrate or substrate/modifier ratios during kinetic experiments.

    Related Experiment Videos

    Main Results:

    • Viewing complex formation as ion exchange/substitution offers practical advantages.
    • Surrogate ligands effectively reveal details of complex formation and dissociation.
    • Constant ratio methods yield linear kinetic plots for rigorous evaluation.

    Conclusions:

    • Enzyme-ligand complexation can be effectively modeled as ion exchange or substitution.
    • This approach enhances the study of enzyme reaction mechanisms.
    • Simplified kinetic analysis improves the reliability of experimental data interpretation.