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Related Experiment Videos

Polyacrylamide gel electrophoresis patterns produced by Clostridium difficile.

H Wexler, M E Mulligan, S M Finegold

    Reviews of Infectious Diseases
    |March 1, 1984
    PubMed
    Summary

    Polyacrylamide gel electrophoresis (PAGE) of Clostridium difficile protein profiles can aid in hospital-acquired infection studies. This method effectively distinguishes C. difficile from C. sordellii, offering a valuable tool for tracking infections.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Epidemiology

    Background:

    • Clostridium difficile is a significant cause of pseudomembranous colitis, often acquired in hospital settings after antibiotic treatment.
    • Understanding the epidemiology of C. difficile is crucial for infection control and prevention strategies.

    Purpose of the Study:

    • To evaluate the utility of Polyacrylamide Gel Electrophoresis (PAGE) analysis of Clostridium difficile protein profiles for epidemiologic investigations.
    • To differentiate C. difficile strains from each other and from related species like Clostridium sordellii using protein profiling.

    Main Methods:

    • Soluble cell extracts of C. difficile strains were analyzed using PAGE, resolving at least 50 protein bands.
    • Protein profiles were assessed for stability against freeze-thawing and storage duration.

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  • Surface antigens and Ethylenediaminetetraacetate (EDTA) extracts were also analyzed by PAGE for comparative purposes.

    • Main Results:

    • PAGE analysis revealed highly conserved protein profiles across most C. difficile strains, with a few unique variations noted (e.g., absence of a 34,000-dalton polypeptide).
    • Distinct protein patterns clearly differentiated C. difficile from Clostridium sordellii.
    • EDTA extracts from C. difficile showed subgroupings based on PAGE band distribution, while C. sordellii extracts were dissimilar.

    Conclusions:

    • PAGE analysis of C. difficile protein profiles is a viable method for epidemiologic studies, particularly for distinguishing it from C. sordellii.
    • The technique can potentially aid in tracking hospital-acquired C. difficile infections by characterizing strain variations.