Related Experiment Videos
Hypercoagulation and fibrinolysis in oral sub-mucous fibrosis
Abstract:
In seven out of seven patients suffering from oral submucous fibrosis (OSMF), we detected circulating molecules that are immunologically similar to fibrinogen (MISFI), as suggested by the hemagglutination inhibition studies using the FDP Kit (Wellcome) and by paracoagulation tests such as serial dilution protamine tests. The ethanol gelation test was positive in three of the seven patients. Cryofibrinogen developed in six of the seven patients within three to six days of incubation of plasma samples at 4 degrees C. Plasma kept at 37 degrees C did not develop any fibrous or amorphous precipitate. In OSMF, fibrinogen, fibrinogen intermediates, and fibrin degradation products deserve further scrutiny, as this may help define the etiology of OSMF which is, so far, obscure.
Insights
Circulating molecules similar to fibrinogen were found in all oral submucous fibrosis (OSMF) patients. Further research into these fibrinogen-related products may clarify the obscure etiology of OSMF.
Area of Science:
- Biochemistry
- Oral Pathology
- Hematology
Background:
- Oral submucous fibrosis (OSMF) is a chronic, potentially malignant oral disorder.
- The etiology of OSMF remains largely unknown.
- Abnormalities in hemostasis and fibrinolysis have been implicated in fibrotic diseases.
Purpose of the Study:
- To investigate the presence and characteristics of fibrinogen-related molecules in patients with OSMF.
- To explore potential links between fibrinogen abnormalities and the pathogenesis of OSMF.
Main Methods:
- Hemagglutination inhibition studies using the FDP Kit (Wellcome).
- Paracoagulation tests including serial dilution protamine tests and ethanol gelation tests.
- Cryofibrinogen precipitation assays after plasma incubation at 4°C and 37°C.
Main Results:
- Molecules immunologically similar to fibrinogen (MISFI) were detected in all seven OSMF patients.
- The ethanol gelation test was positive in three patients.
- Cryofibrinogen developed in six of seven patients upon cold incubation (4°C) of plasma.
- No precipitate formed in plasma incubated at 37°C.
Conclusions:
- Patients with OSMF exhibit circulating fibrinogen-like molecules.
- Fibrinogen, its intermediates, and fibrin degradation products warrant further investigation in OSMF.
- Understanding these molecular alterations may contribute to elucidating the etiology of OSMF.