Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

General method for determining anaerobic biodegradation potential.

D R Shelton, J M Tiedje

    Applied and Environmental Microbiology
    |April 1, 1984
    PubMed
    Summary

    A new method accurately assesses organic chemical anaerobic biodegradation using sewage sludge. This reliable technique identifies chemicals degradable to methane and carbon dioxide, aiding environmental safety assessments.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Implications of direct amplification for measuring antimicrobial resistance using point-of-care devices.

    Analytical methods : advancing methods and applications·2018
    Same author

    Draft genome of the fungicidal biological control agent Burkholderia anthina strain XXVI.

    Archives of microbiology·2018
    Same author

    Differential release of manure-borne bioactive phosphorus forms to runoff and leachate under simulated rain.

    Journal of environmental management·2017
    Same author

    Depth-Dependent Survival of Escherichia coli and Enterococci in Soil after Manure Application and Simulated Rainfall.

    Applied and environmental microbiology·2015
    Same author

    Performance of Weibull and Linear Semi-logarithmic Models in Simulating Inactivation in Waters.

    Journal of environmental quality·2015
    Same author

    Response of coliform populations in streambed sediment and water column to changes in nutrient concentrations in water.

    Water research·2014

    Area of Science:

    • Environmental microbiology
    • Biotechnology
    • Chemical analysis

    Background:

    • Assessing anaerobic biodegradation is crucial for environmental risk evaluation.
    • Existing methods may lack generalizability or efficiency.
    • Standardized testing is needed for organic chemical assessment.

    Purpose of the Study:

    • To refine and validate a generalized method for testing anaerobic biodegradation of organic chemicals.
    • To establish criteria for classifying chemicals as degradable or partially degradable.
    • To develop a reliable and cost-effective assay for biodegradation potential.

    Main Methods:

    • Utilized digested sewage sludge incubated anaerobically in serum bottles.
    • Measured biodegradation by gas pressure increase (CH4 + CO2) via gas chromatography or pressure transducer.
    • Developed a revised anaerobic salts medium and identified reference chemicals (p-cresol, phthalic acid, ethanol).

    Main Results:

    • The pressure transducer method is recommended for speed, accuracy, and cost-effectiveness.
    • Suitable sludge can be stored anaerobically at 4°C for up to 4 weeks.
    • Over 100 chemicals were tested, with 46 found to be anaerobically degraded, demonstrating reproducible results.

    Conclusions:

    • The validated method reliably determines the anaerobic biodegradation potential of organic chemicals.
    • The method is applicable to diverse chemicals and various municipal sludges.
    • This assay provides a robust tool for environmental safety and chemical assessment.

    Related Experiment Videos