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Molecular homology among the structural proteins of densonucleosis virus from silkworm, Bombyx mori

H Bando, N Kondo, S Kawase

    Archives of Virology
    |January 1, 1984
    PubMed

    Insights

    Four Bombyx densonucleosis virus (DNV) structural proteins share sequence homology and common antigens, suggesting a common origin. VP2 is not a direct cleavage product of VP3.

    Area of Science:

    • Virology
    • Molecular Biology
    • Protein Chemistry

    Background:

    • Bombyx densonucleosis virus (DNV) possesses four distinct structural proteins.
    • Understanding the relationship between these structural proteins is crucial for deciphering DNV assembly and replication.

    Purpose of the Study:

    • To investigate the similarities among the four structural proteins of Bombyx DNV.
    • To determine if these proteins share sequence homology, common antigens, and similar amino acid compositions.
    • To elucidate the potential origin and relationship between the structural proteins, specifically VP2 and VP3.

    Main Methods:

    • Peptide mapping using Staphylococcus aureus V8 protease and chymotrypsin.
    • Immunodiffusion tests to detect shared antigens.
    • Amino acid analysis to compare compositional similarities.
    • Enzyme-linked immunosorbent assay (ELISA) technique.

    Main Results:

    • Peptide mapping revealed sequence homology among the four structural proteins.
    • Immunodiffusion tests confirmed the presence of common antigens shared by these proteins.
    • Amino acid analysis showed significant compositional similarities.
    • Discrepancies in specific amino acid residues (e.g., serine) between VP3 and VP2 suggest VP2 is not a direct cleavage product of VP3.

    Conclusions:

    • The four structural proteins of Bombyx DNV likely originate, at least partially, from a common DNA sequence.
    • Evidence supports a shared evolutionary or biosynthetic pathway for these proteins.
    • The structural protein VP2 is not a direct cleavage product of VP3, indicating distinct genetic origins or processing events.

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