Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rhodopsin lateral diffusion as a function of rod outer segment disk membrane axial position.

M W Kaplan

    Biophysical Journal
    |April 1, 1984
    PubMed
    Summary

    Rhodopsin pigment mobility slows slightly with disk age in frog outer segments. This suggests subtle changes in membrane composition may affect rhodopsin diffusion over time.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Exposure of rod outer segments to serum is not responsible for abnormal disk membrane morphogenesis in a model of retinal detachment.

    Current eye research·1998
    Same author

    Disk membrane initiation and insertion are not required for axial disk displacement in Xenopus laevis rod outer segments.

    Current eye research·1998
    Same author

    Characterization of a truncated form of arrestin isolated from bovine rod outer segments.

    Protein science : a publication of the Protein Society·1994
    Same author

    Mechanism of rhodopsin kinase activation.

    The Journal of biological chemistry·1991
    Same author

    Retinal detachment prevents normal assembly of disk membranes in vitro.

    Investigative ophthalmology & visual science·1990
    Same author

    Temperature-dependent birefringence patterns in Xenopus rod outer segments.

    Experimental eye research·1989

    Area of Science:

    • Biophysics
    • Cell Biology
    • Photoreceptor Physiology

    Background:

    • Rhodopsin is the primary visual pigment in photoreceptor cells.
    • Understanding rhodopsin's movement is crucial for visual signal transduction.
    • The structure and dynamics of the photoreceptor membrane influence pigment function.

    Purpose of the Study:

    • To investigate the lateral diffusion of rhodopsin in frog outer segments.
    • To determine if rhodopsin mobility varies along the axis of the outer segment.
    • To explore the relationship between disk age and pigment mobility.

    Main Methods:

    • Utilized absorbance recovery after photobleaching to measure rhodopsin diffusion.
    • Quantified recovery times at three distinct locations along the outer segment axis.
    • Analyzed diffusion rates in both proximal and distal disk membranes.

    Main Results:

    • Mean recovery times for rhodopsin diffusion were longer in distal disk membranes compared to proximal disks.
    • A slight reduction in pigment mobility was observed with increasing disk age.
    • These findings indicate a potential age-dependent change in membrane properties.

    Conclusions:

    • Rhodopsin lateral diffusion is not uniform across the frog outer segment.
    • Pigment mobility may decrease as disk membranes age.
    • Subtle alterations in membrane composition could underlie the observed changes in rhodopsin mobility.

    Related Experiment Videos