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UV-induced small structural changes in the T7 bacteriophage studied by melting methods.

K Tóth, J Bolard, G Rontó

    Biophysics of Structure and Mechanism
    |January 1, 1984
    PubMed
    Summary

    UV irradiation damages T7 bacteriophage structure, affecting its biological activity. Structural destabilization, indicated by altered melting curves, correlates with UV-induced DNA damage.

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    Area of Science:

    • Structural biology
    • Photochemistry
    • Virology

    Background:

    • UV irradiation can cause biological inactivation of viruses like T7 bacteriophage.
    • Assessing structural damage is crucial for understanding viral inactivation mechanisms.

    Purpose of the Study:

    • To investigate UV-induced structural damages in T7 bacteriophage using optical absorption and circular dichroism (CD).
    • To correlate structural changes with biological inactivation and DNA damage.

    Main Methods:

    • UV irradiation of T7 bacteriophage at doses of 0.2-1.2 kJ/m2 (254 nm).
    • Analysis of UV optical absorption and circular dichroism (CD) spectra.
    • Measurement of melting curves to assess helix-coil transition stability.

    Main Results:

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    • UV irradiation led to decreased melting temperature and broadened helix-coil transition range, indicating structural destabilization.
    • These changes suggest the presence of approximately 0.1-0.6% damaged base concentration.
    • Structural damage correlated with initial genetic damage observed at minimal effective UV doses.

    Conclusions:

    • UV irradiation causes significant structural destabilization in T7 bacteriophage.
    • Optical absorption and CD are effective methods for detecting UV-induced structural damage in situ.
    • Structural integrity is linked to the biological activity and UV resistance of T7 bacteriophage.