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Related Experiment Videos

[Androgen binding by skeletal muscle nuclei].

M G Stepanov, B I Fel'dkoren

    Biulleten' Eksperimental'Noi Biologii I Meditsiny
    |May 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

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    Researchers identified specific androgen receptors in rat skeletal muscle nuclei. These receptors are proteins that bind to 3H-19-nortestosterone, with DNA stabilizing their structure.

    Area of Science:

    • Endocrinology
    • Molecular Biology
    • Biochemistry

    Context:

    • Investigates the binding characteristics of androgens in rat skeletal muscle.
    • Focuses on the interaction of 3H-19-nortestosterone with isolated nuclei and nuclear extracts.

    Purpose:

    • To characterize the binding affinity and capacity of androgen receptors in rat skeletal muscle.
    • To determine the proteinaceous nature of nuclear androgen receptors and the influence of DNA.

    Summary:

    • Specific binding of 3H-19-nortestosterone to rat skeletal muscle nuclei and 0.4 M KCl nuclear extracts was observed.
    • Dissociation constants (Kd) were measured at approximately 11.6 nM and 9.9 nM, respectively.
    • Nuclear androgen receptors were confirmed as proteins through enzymatic digestion, with DNA exhibiting a stabilizing effect.

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    Impact:

    • Provides insights into the molecular mechanisms of androgen action in skeletal muscle.
    • Characterizes androgen receptor properties, crucial for understanding muscle growth and maintenance.
    • Demonstrates that DNase treatment enhances the extraction of nuclear androgen receptors.