Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nuclear area changes in senescing human diploid fibroblasts.

S C Lee, P M Bemiller, J N Bemiller

    Mechanisms of Ageing and Development
    |June 1, 1978
    PubMed
    Summary

    Human fibroblast cells showed increased nuclear size during in vitro aging, regardless of donor age. Larger nuclei correlated with both in vitro aging and the donor

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Bread Staling: Molecular Basis and Control.

    Comprehensive reviews in food science and food safety·2021
    Same author

    Effects of activation frequency on dynamic performance of human fresh and fatigued muscles.

    Journal of applied physiology (Bethesda, Md. : 1985)·2000
    Same author

    Carbon urea breath test is not as accurate as endoscopy to detect Helicobacter pylori after gastrectomy.

    Gastrointestinal endoscopy·2000
    Same author

    Polymorphisms of N-acetyltransferase 2, glutathione S-transferase mu and theta genes as risk factors of bladder cancer in relation to asthma and tuberculosis.

    The Journal of urology·2000
    Same author

    A 5-year study of the seroepidemiology of Klebsiella pneumoniae: high prevalence of capsular serotype K1 in Taiwan and implication for vaccine efficacy.

    The Journal of infectious diseases·2000
    Same author

    Indoor and outdoor air quality investigation at schools in Hong Kong.

    Chemosphere·2000

    Area of Science:

    • Cellular biology
    • Gerontology
    • Biogerontology

    Background:

    • Cellular senescence is a key hallmark of aging.
    • In vitro cell culture models are crucial for studying aging mechanisms.
    • Nuclear morphology changes are associated with cellular aging.

    Purpose of the Study:

    • To investigate changes in mean nuclear area of human fibroblasts during in vitro aging.
    • To determine if donor age influences nuclear area changes during cellular aging.
    • To explore the correlation between in vitro aging and donor age.

    Main Methods:

    • Human fibroblast cell strains from male donors of three different ages (8, 40, and 84 years) were cultured in vitro.
    • Mean nuclear area was measured throughout the in vitro lifespan of each cell strain.
    • Statistical analysis was performed to assess changes in nuclear area and correlations.

    Main Results:

    • A statistically significant increase in mean nuclear area was observed in all three fibroblast strains during in vitro aging.
    • A gradual increase in the subpopulation of cells with larger nuclei was noted.
    • A positive correlation was found between in vitro aging (reflected by nuclear area) and donor age.

    Conclusions:

    • In vitro aging of human fibroblasts is characterized by an increase in mean nuclear area.
    • Donor age is positively correlated with the extent of in vitro aging as measured by nuclear area.
    • Nuclear size serves as a potential biomarker for cellular aging and its relationship with organismal age.

    Related Experiment Videos