Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reductive halothane metabolite formation and halothane binding in rat hepatic microsomes.

M T Baker, R A Van Dyke

    Chemico-Biological Interactions
    |April 1, 1984
    PubMed
    Summary

    This study investigated halothane metabolism, identifying key reductive metabolites 2-chloro-1,1,1-trifluoroethane (CTE) and 2-chloro-1,1-difluoroethylene (CDE). Results show factors influencing their production and halothane binding, crucial for understanding anesthetic drug metabolism.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Standardization of doctoral study in agricultural and extension education: is the field of study mature enough for achievement of the optimum degree of order?

    Communications in agricultural and applied biological sciences·2010
    Same author

    Pharmacological effects of intravenous melatonin: comparative studies with thiopental and propofol.

    British journal of anaesthesia·2003
    Same author

    Yellowing of metabisulfite-containing propofol emulsion.

    American journal of health-system pharmacy : AJHP : official journal of the American Society of Health-System Pharmacists·2001
    Same author

    Comparative propofol emulsion stabilities.

    AANA journal·2000
    Same author

    Not to be and then to be: visual representation of ignored unfamiliar faces.

    Journal of experimental psychology. Human perception and performance·2000
    Same author

    The Reasons for Living Inventory for Adolescents (RFL-A): development and psychometric properties.

    Journal of clinical psychology·1998

    Area of Science:

    • Biochemistry
    • Pharmacology
    • Toxicology

    Background:

    • Halothane is an anesthetic agent with known reductive metabolites.
    • Understanding the metabolic pathways and factors influencing halothane degradation is critical for patient safety.

    Purpose of the Study:

    • To quantify the production of reductive [14C]halothane metabolites, 2-chloro-1,1,1-trifluoroethane (CTE) and 2-chloro-1,1-difluoroethylene (CDE).
    • To investigate the influence of reducing agents (NADPH, sodium dithionite) and other anesthetics (isoflurane, enflurane) on metabolite production and halothane binding.
    • To correlate metabolite formation with fluoride release and halothane-lipid binding.

    Main Methods:

    • Anaerobic microsomal incubations were employed to study halothane metabolism.
    • High-performance liquid chromatography (HPLC) was utilized for accurate quantification of [14C]halothane metabolites.

    Related Experiment Videos

  • Fluoride levels were measured to assess metabolic breakdown.

    • Main Results:

    • Sodium dithionite stimulated CDE production and halothane degradation but inhibited CTE formation and binding.
    • Isoflurane increased CDE production while decreasing CTE formation and halothane-lipid binding.
    • Fluoride release correlated with CDE formation, not CTE formation.

    Conclusions:

    • The relative production of CTE and CDE is variable in microsomal preparations.
    • Halothane binding is associated with CTE formation, independent of CDE and fluoride production.