Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Indophenol method for acetaminophen in serum examined.

P E Novotny, R C Elser

    Clinical Chemistry
    |June 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Double modulation-optical scanning and mechanical chopping-in atomic absorption spectrometry using a continuum source.

    Analytical chemistry·2012
    Same author

    Potential effects of age-specific reference ranges for serum prostate-specific antigen.

    European urology·1995
    Same author

    International Federation of Clinical Chemistry, Scientific Division Committee on Enzymes: approved recommendation on IFCC methods for the measurement of catalytic concentration of enzymes. Part 7. IFCC method for creatine kinase (ATP: creatine N-phosphotransferase, EC 2.7.3.2).

    European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies·1991
    Same author

    Chemical control of reaction time in an enzyme assay and feasibility of enzyme spot tests.

    Analytical chemistry·1990
    Same author

    International Federation of Clinical Chemistry. Scientific Division, Committee on Enzymes. IFCC methods for the measurement of catalytic concentration of enzymes. Part 7. IFCC method for creatine kinase (ATP: creatine N-phosphotransferase, EC 2.7.3.2). IFCC recommendations.

    Clinica chimica acta; international journal of clinical chemistry·1990
    Same author

    International Federation of Clinical Chemistry (IFCC): Scientific Division, Committee on Enzymes. IFCC methods for the measurement of catalytic concentration of enzymes. Part 7. IFCC method for creatine kinase (ATP: creatine (N-phosphotransferase, EC 2.7.3.2). IFCC Recommendation.

    The Journal of automatic chemistry·1990
    Same journal

    From Pooled Cohorts to PREVENT: A Perspective for Clinical Laboratorians.

    Clinical chemistry·2026
    Same journal

    Interlaboratory Comparison of a Glucagon and Oxyntomodulin Immuno-LC-MS/MS Assay: Implications for Diabetes Research.

    Clinical chemistry·2026
    Same journal

    Comparison of Information-Dependent Acquisition and Sequential Window Acquisition of All Theoretical Mass Spectra for Untargeted Drug Testing on a Linear Ion Trap-Pulsing Quadrupole-Time of Flight Mass Spectrometer.

    Clinical chemistry·2026
    Same journal

    Patterns of One-Year Change in HbA1c and Continuous Glucose Monitoring (CGM) Metrics in Older Adults with Type 2 Diabetes.

    Clinical chemistry·2026
    Same journal

    TSH Pediatric Reference Intervals: Lack of CALIPER Applicability to US-Based Populations.

    Clinical chemistry·2026
    Same journal

    Rapid Detection of Hemoglobinopathy Variants Using One-Step Library Preparation and Nanopore Sequencing.

    Clinical chemistry·2026
    See all related articles

    This study enhances a colorimetric method for acetaminophen detection by optimizing hydrolysis and color development. The improved assay offers linear correlation to 250 mg/L and is unaffected by uremic patient serum compounds.

    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Toxicology

    Background:

    • The indophenol-based colorimetric method is commonly used for acetaminophen quantification.
    • Previous studies indicated potential limitations in the sensitivity of this method.

    Purpose of the Study:

    • To identify and address the factors limiting the sensitivity of the indophenol-based acetaminophen assay.
    • To optimize the existing colorimetric method for improved accuracy and reliability.
    • To validate the modified method's performance, including its linearity and interference-free nature.

    Main Methods:

    • Investigated suboptimal acid concentration and hydrolysis time affecting incomplete hydrolysis.
    • Assessed potential reagent deterioration of ammonium hydroxide over time.

    Related Experiment Videos

  • Optimized hydrolysis duration and color development time for the assay.
  • Evaluated the modified method's performance against known acetaminophen concentrations.
  • Tested the method's specificity using serum samples from uremic patients.

    • Main Results:

    • Identified incomplete hydrolysis due to suboptimal acid concentration and hydrolysis time as key limitations.
    • Observed potential deterioration of the ammonium hydroxide reagent after 2.5 months.
    • Achieved linear correlation between results and acetaminophen concentrations up to 250 mg/L (1.66 mmol/L).
    • Demonstrated that the modified method is free from interference by serum compounds in uremic patients.

    Conclusions:

    • The original indophenol-based method for acetaminophen has suboptimal sensitivity.
    • Optimizing hydrolysis and color development significantly improves the assay's performance.
    • The modified method provides a reliable and sensitive tool for acetaminophen quantification, even in the presence of interfering substances found in uremic serum.