Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protamines in plant sperm.

W F Reynolds, S L Wolfe

    Experimental Cell Research
    |June 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Plant sperm protamines from algae, bryophytes, and ferns were analyzed. These arginine-rich proteins show similarities, suggesting a conserved role in plant male gamete DNA packaging.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Reaction of dibenzalacetone with hydroxylamine hydrochloride under phase-transfer conditions. <sup>1</sup> H and <sup>13</sup> C 2D NMR structural elucidation of the products.

    Magnetic resonance in chemistry : MRC·2021
    Same author

    Thiocyanate supplementation decreases atherosclerotic plaque in mice expressing human myeloperoxidase.

    Free radical research·2015
    Same author

    Applying computer-assisted structure elucidation algorithms for the purpose of structure validation: revisiting the NMR assignments of hexacyclinol.

    Journal of natural products·2008
    Same author

    The isolation of soyasaponins by fractional precipitation, solid phase extraction, and low pressure liquid chromatography.

    International journal of food sciences and nutrition·2006
    Same author

    Constituents of Agave americana and Agave barbadensis.

    Fitoterapia·2005
    Same author

    Novel bis(monoterpenoid) indole alkaloids from Psychotria bahiensis.

    Journal of natural products·2003
    Same journal

    The AP2α/PDHA1 signaling pathway promotes lung cancer progression by mediating aerobic glycolysis and inhibiting cuproptosis.

    Experimental cell research·2026
    Same journal

    Distributed transcription activity in DLX proteins defies conventional mapping of a transcription activation domain.

    Experimental cell research·2026
    Same journal

    Extracellular release of cytotoxic aggregates from HSV-1-replicating SH-SY5Y cells: involvement of alpha-synuclein and poly-ubiquitin conjugates.

    Experimental cell research·2026
    Same journal

    MiR-1281 downregulates LMX1B to inhibit gastric cancer development.

    Experimental cell research·2026
    Same journal

    Corrigendum to "Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells" [Exp. Cell Res., 326, 1, 1 August 2014, Pages 78-89].

    Experimental cell research·2026
    Same journal

    Neutrophilic differentiation of promyelocytic HL-60 cells is associated with increased expression of terminal galactose residues on the cell surface.

    Experimental cell research·2026
    See all related articles

    Area of Science:

    • Plant biology
    • Molecular biology
    • Biochemistry

    Background:

    • Sperm protamines are essential for DNA condensation in male gametes.
    • Histones are typically displaced by protamines during sperm development.
    • Previous work established histone displacement by protamines in Marchantia.

    Purpose of the Study:

    • To isolate and characterize sperm protamines from diverse plant groups.
    • To compare the properties of protamines from algae, bryophytes, and ferns.

    Main Methods:

    • Isolation of sperm protamines from Chara corallina (algae), Marchantia polymorpha (bryophyte), and Marsilea vestitia (fern).
    • Analysis of protamine components using acid-urea gel electrophoresis.
    • Determination of amino acid composition and molecular weights.

    Related Experiment Videos

    Main Results:

    • Protomines were successfully isolated from all three plant groups.
    • Marchantia protamines resolved into four components, while Chara and Marsilea protamines appeared as a single band.
    • Plant protamines are arginine-rich (35-42% basic amino acids) and exhibit high amino acid composition similarity (84-91%).
    • Molecular weights for Chara and Marsilea protamines were estimated at 4700-5300 D.

    Conclusions:

    • Plant sperm protamines are conserved, arginine-rich proteins.
    • These findings highlight a common mechanism for DNA packaging in plant male gametes across different lineages.
    • Protomines play a fundamental role in chromatin condensation during plant spermatogenesis.