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Extrachromosomal circular DNAs in interspecific mouse-rat reconstituted cells
Gan
|January 1, 1984
Summary
Reconstituted cells formed from mouse and rat cells showed larger circular DNA initially, which was lost over time. These findings suggest rat cytoplasts may induce DNA rearrangements in mouse cells.
Area of Science:
- Cell biology
- Genetics
- Molecular biology
Background:
- Extrachromosomal circular DNA exists in mammalian cells.
- Cell fusion techniques allow for the creation of reconstituted cells with mixed genetic material.
Purpose of the Study:
- To investigate the presence and behavior of extrachromosomal circular DNA in reconstituted cells.
- To explore the stability of new phenotypes and the potential role of cytoplasts in DNA rearrangement.
Main Methods:
- Fusion of mouse melanoma B16 karyoplasts with rat myoblastic L6 cytoplasts.
- Isolation of reconstituted cell clones using the mica-press-adsorption method.
- Electron microscopy for visualizing extrachromosomal DNA.
Main Results:
- Both parental cells contained smaller circular DNA (<1 micron).
- Reconstituted cells initially showed more frequent larger circular DNA (>1 micron).
- New cell phenotypes were stable, but larger circular DNA diminished with prolonged cultivation.
Conclusions:
- Larger circular DNA may arise from intrachromosomal DNA rearrangement induced by rat cytoplasts.
- The presence and stability of extrachromosomal DNA are influenced by cell fusion and subsequent cultivation.