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Related Experiment Videos

Filtration coefficient obtained by stepwise pressure elevation in isolated dog lung.

I C Ehrhart, W M Granger, W F Hofman

    Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology
    |April 1, 1984
    PubMed
    Summary

    This study shows that measuring lung capillary filtration coefficient (Kf) using stepwise pressure elevation in isolated lungs is reliable. This method accurately quantifies increased vascular permeability after lung injury.

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    Area of Science:

    • Pulmonary Physiology
    • Cardiovascular Research
    • Lung Injury Models

    Background:

    • The capillary filtration coefficient (Kf) is a key indicator of lung vascular permeability.
    • Previous studies suggest a higher baseline Kf in isolated versus intact dog lungs.
    • The stepwise pressure elevation technique requires further validation for Kf determination.

    Purpose of the Study:

    • To evaluate the stepwise pressure elevation technique for measuring baseline Kf in isolated dog lungs.
    • To assess changes in Kf following oleic acid-induced lung injury.
    • To determine the utility of this technique for quantifying alterations in pulmonary vascular permeability.

    Main Methods:

    • Isolated left lower lung lobes from dogs were ventilated and pump-perfused.

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  • The stepwise pressure elevation technique was used to measure lobe weight gain.
  • Control (saline infusion) and injured (oleic acid infusion) groups were compared.

    • Main Results:

    • Baseline Kf in control isolated lung lobes was 0.071 +/- 0.012 ml x min-1 x Torr-1 x 100 g-1.
    • Kf increased significantly to 0.243 +/- 0.027 ml x min-1 x Torr-1 x 100 g-1 in oleic acid-injured lobes.
    • The technique yielded baseline Kf values comparable to other gravimetric methods.

    Conclusions:

    • The stepwise pressure elevation technique provides a reliable baseline Kf measurement in isolated lungs.
    • This method effectively quantifies the substantial increase in vascular permeability post-lung injury.
    • The technique is a valuable tool for studying pulmonary vascular permeability changes.