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Related Experiment Videos

Titrimetric determination of ascorbic acid using chloranil.

K K Verma, A Jain, R Rawat

    Journal - Association of Official Analytical Chemists
    |March 1, 1984
    PubMed
    Summary

    This study presents a new method for quantifying ascorbic acid using chloranil titration. The technique is accurate and effective even in the presence of interfering substances.

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    Area of Science:

    • Analytical Chemistry
    • Biochemistry

    Background:

    • Ascorbic acid (vitamin C) is an essential nutrient with various biological roles.
    • Accurate quantification of ascorbic acid is crucial for nutritional analysis and quality control.

    Purpose of the Study:

    • To develop and validate a novel quantitative titration method for ascorbic acid.
    • To assess the method's reliability in complex matrices and identify potential interferences.

    Main Methods:

    • Titration of ascorbic acid with chloranil (2,3,5,6-tetrachloro-1,4-benzoquinone) in an acetone-water solvent system.
    • Visual endpoint detection via a golden yellow color change.
    • Spectrophotometric analysis at 451 nm for colored solutions, plotting absorbance against titrant volume.
    • Masking strategies using acrylamide for thiol interference and ammonium thiocyanate/sodium potassium tartrate for iron(II).
    • Inclusion of antioxidants like hydrogen sulfite and thiourea during sample extraction.

    Main Results:

    • The chloranil titration method provides quantitative results for ascorbic acid.
    • Spectrophotometric analysis allows for accurate endpoint determination in colored solutions.
    • Effective masking agents were identified to mitigate interference from thiol compounds and iron(II).
    • Antioxidants ensure ascorbic acid stability during extraction from diverse sources.

    Conclusions:

    • The described chloranil titration method offers a reliable and accurate approach for ascorbic acid quantification.
    • The method is robust, accommodating various sample types and mitigating common interfering substances.
    • This technique provides a valuable tool for the precise determination of ascorbic acid in pharmaceutical and food samples.

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