Comparison of two superfusion systems for study of neurotransmitter release from rat cerebral cortex slices

Depolarization-induced release of [3H]gamma-aminobutyric acid (GABA) and [3H]noradrenaline (NA) from rat cerebral cortex slices was studied in two superfusion systems: one with stationary and the other one with continuously shaken slice compartments. Calcium-dependent depolarization-induced release of GABA and NA could be demonstrated only with shaken slices. GABA, but not NA, could also be released by high K+ media and veratridine from stationary slices. Synaptic transmitter releasing mechanisms are apparently damaged in stationary slices, possibly due to impaired energy metabolism.