Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Preliminary crystallographic data for exfoliative toxin B from Staphylococcus aureus.

D Moras, J C Thierry, J Cavarelli

    Journal of Molecular Biology
    |May 5, 1984
    PubMed
    Summary

    Researchers crystallized serotype B exfoliative toxin from Staphylococcus aureus. This structural study provides insights into the toxin

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A suite of macrocyclic peptide inhibitors and substrate probes for arginine methyltransferases.

    Chemical science·2026
    Same author

    Ligand selectivity by nuclear hormone receptors.

    Journal of biomolecular structure & dynamics·2012
    Same author

    Aminoacylation at the Atomic Level in Class IIa Aminoacyl-tRNA Synthetases.

    Journal of biomolecular structure & dynamics·2012
    Same author

    Evaluation of a new chromogenic medium, chromID Vibrio, for the isolation and presumptive identification of Vibrio cholerae and Vibrio parahaemolyticus from human clinical specimens.

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2011
    Same author

    Mycobacterium avium-intracellulare contamination of mammalian cell cultures.

    In vitro cellular & developmental biology. Animal·2008
    Same author

    [Community-acquired methicillin-resistant Staphylococcus aureus in a dermatology outpatient clinic].

    Annales de dermatologie et de venereologie·2008

    Area of Science:

    • Crystallography
    • Structural Biology
    • Microbiology

    Background:

    • Staphylococcus aureus produces exfoliative toxins responsible for skin blistering diseases.
    • Understanding the structure of these toxins is crucial for developing targeted interventions.

    Purpose of the Study:

    • To crystallize and determine the structural characteristics of serotype B exfoliative toxin from Staphylococcus aureus strain TC 142.

    Main Methods:

    • Isolation and purification of Staphylococcus aureus strain TC 142.
    • Crystallization of the purified serotype B exfoliative toxin.
    • X-ray diffraction analysis to determine crystal structure and space group.

    Main Results:

    • Serotype B exfoliative toxin was successfully crystallized.

    Related Experiment Videos

  • The crystals belong to the monoclinic space group P21.
  • Unit cell dimensions and the presence of two molecules in the asymmetric unit were determined.

    • Conclusions:

    • The successful crystallization of serotype B exfoliative toxin provides a foundation for detailed structural analysis.
    • This structural information can aid in understanding the toxin's mechanism of action.
    • Further studies can explore structure-based drug design for Staphylococcus aureus infections.