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Updated: Aug 12, 2026

The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion
Published on: November 30, 2022
Hepatic lymph flow and protein concentration during intravenous saline infusion and intestinal fluid absorption
Abstract:
A simple procedure for the collection of the postnodal and prenodal hepatic lymph in the rats under pentobarbital anesthesia is described. Lymph flow (JL) from the main hepatic lymph duct ( postnodal ), protein concentration in both the lymph (CL) and plasma (CP), and the CL/CP ratio were 0.22 +/- 0.1 ml/kg of body weight . hr (0.66 +/- 0.3 ml/100 g wet liver wt . hr), 4.3 +/- 0.5 and 6.2 +/- 0.3 g%, and 0.69 +/- 0.1 (mean +/- SE), respectively. There was no significant difference in CL between prenodal and postnodal lymph in the fasted or unfasted rats. During intestinal fluid absorption with an increase in intestinal JL, postnodal hepatic JL or CL did not change. During intravenous saline infusion with lowering of plasma oncotic pressure, there was a small increase in prenodal JL (by approximately 160%) with some decrease (by 16%) in CL/CP ratio but a much larger increase in postnodal JL (by approximately 700%) with a significant decrease (by 55%) in CL/CP ratio. The results demonstrate that both flow and protein concentration of the postnodal lymph differ greatly from that of the prenodal lymph due to modification during transit through the lymph node. The small increase of the prenodal JL with negligible change in CL/CP ratio during saline infusion suggests that oncotic pressure grandient plays a minor or insignificant role in the transsinusoidal fluid transport in the liver.
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