Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new automated technique for platelet aggregation measurement.

H K Nielsen, N J Christensen, S E Husted

    Scandinavian Journal of Clinical and Laboratory Investigation
    |May 1, 1984
    PubMed
    Summary

    A Multistat III Centrifugal Analyser effectively measures platelet aggregation, showing a 50-75% reduction after low-dose aspirin (ASA) intake. This method is suitable for monitoring ASA treatment to determine optimal prophylactic doses for arterial thromboembolic disease.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Dipyridamole and Platelet Release of Platelet-derived Growth Factor.

    Platelets·2010
    Same author

    Incomplete thromboxane inhibition with 100 mg of intravenous acetylsalicylic acid in patients with acute ST elevation myocardial infarction: a placebo-controlled pilot trial.

    Thrombosis research·2001
    Same author

    Fractures in patients with primary idiopathic hypothyroidism.

    Thyroid : official journal of the American Thyroid Association·2000
    Same author

    [Anticoagulant treatment].

    Ugeskrift for laeger·2000
    Same author

    On the occurrence of anoxic microniches, denitrification, and sulfate reduction in aerated activated sludge.

    Applied and environmental microbiology·1999
    Same author

    Effects of short-term treatment with prednisolone and calcitriol on bone and mineral metabolism in normal men.

    Bone·1998

    Area of Science:

    • Hematology
    • Clinical Chemistry
    • Biomedical Engineering

    Background:

    • Platelet aggregation is crucial in arterial thromboembolic disease.
    • Accurate monitoring of antiplatelet therapy, such as with acetylsalicylic acid (ASA), is essential for prophylaxis.
    • Existing methods for platelet aggregation measurement may have limitations in routine clinical settings.

    Purpose of the Study:

    • To evaluate the efficacy of the Multistat III Centrifugal Analyser (MCA) for measuring platelet aggregation in vitro.
    • To establish the reference range and assess the correlation of MCA results with conventional aggregometry.
    • To determine the utility of the MCA method in monitoring the effects of low-dose acetylsalicylic acid (ASA) treatment.

    Main Methods:

    • Platelet-rich plasma and collagen-reagent were mixed in the MCA.

    Related Experiment Videos

  • Turbidity was measured over time, with results expressed in arbitrary units (arb. units).
  • Correlation with conventional aggregometry (Fibromat) was calculated; platelet aggregation was measured before and after ASA administration.

    • Main Results:

    • The MCA demonstrated a capacity of approximately 40 samples per hour.
    • The estimated 0.95 reference range was 50-95 arb. units (n=46).
    • A high coefficient of correlation (0.85, P<0.001) was found between MCA and conventional methods.
    • MCA registered a 50-75% reduction in platelet aggregation after 3 days of low-dose ASA (1.2-4.0 mg/kg) in 19 subjects.

    Conclusions:

    • The Multistat III Centrifugal Analyser is a reliable and efficient tool for measuring platelet aggregation.
    • The MCA method shows good agreement with conventional aggregometry.
    • This analyser is suitable for routine monitoring of ASA treatment to establish individual appropriate prophylactic ASA doses for arterial thromboembolic disease.