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Related Experiment Videos

Mean platelet volume: the need for a reference method.

G A Threatte, C Adrados, S Ebbe

    American Journal of Clinical Pathology
    |June 1, 1984
    PubMed
    Summary
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    Ethylenediaminetetraacetic acid (EDTA) causes significant, time-dependent artifacts in platelet volume measurements. Buffered citrate solutions offer stable results for up to six hours, preserving accurate platelet analysis.

    Area of Science:

    • Hematology
    • Clinical Pathology
    • Laboratory Medicine

    Background:

    • Accurate platelet volume measurement is crucial for diagnosing and managing various hematological conditions.
    • Artifactual changes in platelet indices can lead to misdiagnosis and inappropriate treatment.
    • Understanding factors influencing platelet measurements is essential for reliable laboratory diagnostics.

    Purpose of the Study:

    • To evaluate the impact of anticoagulants, temperature, and pre-analytical delays on platelet volume measurements.
    • To determine the time course of artifactual effects on platelet indices.
    • To identify optimal collection and handling conditions for stable platelet volume measurements.

    Main Methods:

    • Split blood specimens were analyzed using hydrodynamic focusing.

    Related Experiment Videos

  • Platelet distributions were computed using a least-squares fit to a log-normal distribution.
  • Various anticoagulants (EDTA, buffered citrate, ACD, P-P-P) and storage conditions (temperature, time) were tested.

    • Main Results:

    • Ethylenediaminetetraacetic acid (EDTA) induced extreme, time-dependent artifacts in platelet volume.
    • Cooling specimens to room temperature and delayed anticoagulation also caused significant artifacts.
    • Buffered citrate, Acid Citrate Dextrose (ACD), and Pyridoxal-5'-phosphate (P-P-P) supplemented citrate yielded stable and equivalent results.

    Conclusions:

    • EDTA is unsuitable for accurate, long-term platelet volume measurements due to significant time-dependent artifacts.
    • Buffered citrate, ACD, or P-P-P supplemented citrate, with rapid anticoagulation and 37°C incubation, provide stable platelet volume measurements for up to six hours.
    • Standardizing pre-analytical variables is critical for reliable platelet volume analysis in clinical settings.