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Metabolic activation and deactivation of fusarin C, a mutagen produced by Fusarium moniliforme
Abstract:
The metabolic activation and deactivation of fusarin C, a mutagen produced by Fusarium moniliforme strain MRC 826, was studied by the Salmonella typhimurium mutagenicity assay using tester strain TA 100. A microsomal monooxygenase, preferably induced by phenobarbitone (PB) activities the mutagen to its active mutagenic form. Deactivation of the mutagenic metabolite seems to occur through chemical binding to thiol groups and by enzymatic conjugation mediated by a cytosolic glutathione -S-transferase.
Insights
Fusarin C, a mutagen from Fusarium moniliforme, is activated by microsomal monooxygenase and deactivated by glutathione S-transferase. This study details the metabolic pathways of this fungal toxin.
Area of Science:
- * Mycology
- * Toxicology
- * Biochemistry
Background:
- * Fusarium moniliforme strain MRC 826 produces the mycotoxin fusarin C.
- * Fusarin C is a known mutagen requiring metabolic activation.
- * Understanding its metabolic fate is crucial for risk assessment.
Purpose of the Study:
- * To investigate the metabolic activation and deactivation pathways of fusarin C.
- * To identify the enzymes involved in fusarin C metabolism.
- * To characterize the mutagenicity of fusarin C using a bacterial assay.
Main Methods:
- * Salmonella typhimurium mutagenicity assay with tester strain TA 100.
- * Use of phenobarbitone-induced microsomal monooxygenase for activation studies.
- * Investigation of deactivation mechanisms involving thiol binding and enzymatic conjugation.
Main Results:
- * Microsomal monooxygenase, induced by phenobarbitone, activates fusarin C to a mutagenic form.
- * Deactivation involves chemical binding to thiol groups.
- * Cytosolic glutathione S-transferase mediates enzymatic conjugation for detoxification.
Conclusions:
- * Fusarin C metabolism is a two-step process involving activation and deactivation.
- * Microsomal monooxygenase and glutathione S-transferase play key roles.
- * The findings provide insight into the toxicological mechanisms of fusarin C.