Related Experiment Video
Updated: Aug 14, 2026

In Vivo Immunofluorescence Localization for Assessment of Therapeutic and Diagnostic Antibody Biodistribution in Cancer Research
Published on: September 16, 2019
Abstract:
Immunization of animals for the purpose of generating antisera for clinical and experimental use results in the production of antibodies with multiple specificities directed at the parent antigen. Furthermore, if the immunogen contains contaminants of a highly immunogenic nature, large amounts of irrelevant antibody will be generated and which must subsequently be absorbed. The unpredictable nature of heteroantisera can be overcome by adopting the monoclonal antibody technique which allows one to produce standardized antibody with well defined and selected specificity toward a given epitope on a macromolecule. Cross-reactions are not eliminated by monoclonal antibodies.
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