Related Experiment Video
Updated: Aug 11, 2026

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
High-performance liquid chromatography with column switching for the analysis of biogenic amine metabolites and
Abstract:
An automatic high-performance liquid chromatographic system with facilities for column switching is described which involves minimal pre-treatment of biological samples, separates complex mixtures of compounds in a short period of time and uses fluorimetric or amperometric detection. The system has been applied to the analysis of oxidized pterins in urine and reduced pterins in cerebrospinal fluid and rat brain fractions (R- and S-enantiomers of tetrahydrobiopterin resolved). The system can also be used for the analysis of most of the dopamine and serotonin metabolites in cerebrospinal fluid and brain fractions from norepinephrine to serotonin.
More Related Videos
06:25Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
13:35A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
Published on: March 1, 2018
Related Concept Videos
Principles Of Column Chromatography
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Types of Detectors
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...