Quantitative microradiography for studying dental enamel demineralization and remineralization

A method for quantitative determination of mineral density changes in dental enamel has been developed. It utilizes the quantitative microscopy capabilities of image analysis or photometer systems. Characteristic demineralization of bovine enamel in acetate buffers containing calcium and phosphate has been used to demonstrate the results. The method together with chemical kinetic data obtained from spectrophotometric and ion-selective electrode measurements of the bulk solution for calcium, phosphate, and fluoride permit important basic studies to be conducted on demineralization and remineralization of dental enamel.