Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An automatic pulsed laser microfluorometer with high spatial and temporal resolution.

F Docchio, R Ramponi, C A Sacchi

    Journal of Microscopy
    |May 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Aorto-left ventricular tunnel: a rare cause of heart failure in the newborn.

    Images in paediatric cardiology·2019
    Same author

    Integrated waveguides and deterministically positioned nitrogen vacancy centers in diamond created by femtosecond laser writing.

    Optics letters·2018
    Same author

    Femtosecond laser inscription of Bragg grating waveguides in bulk diamond.

    Optics letters·2017
    Same author

    A novel optical apparatus for the study of rolling contact wear/fatigue based on a high-speed camera and multiple-source laser illumination.

    The Review of scientific instruments·2016
    Same author

    New light in flavin autofluorescence.

    European journal of histochemistry : EJH·2015
    Same author

    Autofluorescence spectroscopy and imaging: a tool for biomedical research and diagnosis.

    European journal of histochemistry : EJH·2015
    Same journal

    In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

    Journal of microscopy·2026
    Same journal

    The future of DXA: How AI is transforming bone health diagnostics.

    Journal of microscopy·2026
    Same journal

    The Origins of Ploem's Filter Cube: A Pandora's Box.

    Journal of microscopy·2026
    Same journal

    The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

    Journal of microscopy·2026
    Same journal

    Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

    Journal of microscopy·2026
    Same journal

    Modular training resources for bioimage analysis.

    Journal of microscopy·2026
    See all related articles

    A new automatic pulsed laser microfluorometer offers high resolution for studying fluorescent molecules. This advanced instrument enables detailed quantitative and qualitative analysis of fluorescence properties.

    Area of Science:

    • Biophysics
    • Analytical Chemistry
    • Optical Engineering

    Background:

    • Fluorescence microscopy is crucial for analyzing molecular properties.
    • High spatial and temporal resolution are essential for accurate fluorescence studies.
    • Existing methods may lack the precision needed for detailed molecular characterization.

    Purpose of the Study:

    • To describe the development of an automatic pulsed laser microfluorometer.
    • To demonstrate the instrument's capability for high-resolution fluorescence analysis.
    • To showcase its application in quantitative and qualitative studies of fluorescent molecules.

    Main Methods:

    • Utilized a nitrogen-laser-pumped dye laser for fluorescence excitation.
    • Integrated a microscope with specialized optics for beam focusing and fluorescence collection.

    Related Experiment Videos

  • Employed filters/monochromators for wavelength selection and a fast photomultiplier tube for signal detection.
  • Implemented a microprocessor-controlled signal averager for data acquisition and processing.

    • Main Results:

    • Successfully developed an automatic pulsed laser microfluorometer.
    • Achieved high spatial and temporal resolution in fluorescence measurements.
    • Demonstrated the instrument's potential through illustrative examples.

    Conclusions:

    • The developed microfluorometer provides a powerful tool for advanced fluorescence studies.
    • Time-resolved fluorescence microscopy enables precise quantitative and qualitative analysis.
    • This instrument facilitates a deeper understanding of fluorescent molecule properties.