Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A radioimmunoassay system for human myoglobin: method development and applications.

L Simionescu, I Radu, L Costache

    Endocrinologie
    |April 1, 1984
    PubMed
    Summary

    A new radioimmunoassay (RIA) system for human myoglobin (hMb) was developed. This assay accurately measures hMb levels in normal subjects and patients with acute myocardial infarction (AMI) or thyroid dysfunction.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The 2021 ultrafast spectroscopic probes of condensed matter roadmap.

    Journal of physics. Condensed matter : an Institute of Physics journal·2021
    Same author

    SURGICAL MANAGEMENT OF THE ADRENAL GLAND TUMORS - SINGLE CENTER EXPERIENCE.

    Acta endocrinologica (Bucharest, Romania : 2005)·2020
    Same author

    Rating the environmental and genetic risk factors for colorectal cancer.

    Journal of medicine and life·2019
    Same author

    THYROID METASTASIS FROM CLEAR CELL CARCINOMA OF THE KIDNEY 16 YEARS AFTER NEPHRECTOMY.

    Acta endocrinologica (Bucharest, Romania : 2005)·2019
    Same author

    IODINE STATUS IN PREGNANT WOMEN AFTER A DECADE OF UNIVERSAL SALT IODIZATION IN ROMANIA.

    Acta endocrinologica (Bucharest, Romania : 2005)·2019
    Same author

    Chemical vapor deposition of monolayer-thin WS<sub>2</sub> crystals from the WF<sub>6</sub> and H<sub>2</sub>S precursors at low deposition temperature.

    The Journal of chemical physics·2019

    Area of Science:

    • Biochemistry
    • Immunology
    • Clinical Chemistry

    Background:

    • Human myoglobin (hMb) is a protein found in muscle tissue.
    • Accurate measurement of hMb is important for diagnosing conditions like acute myocardial infarction (AMI).
    • Existing methods may have limitations in sensitivity or specificity.

    Purpose of the Study:

    • To develop and validate a double antibody radioimmunoassay (RIA) system for quantifying human myoglobin (hMb).
    • To establish the sensitivity, accuracy, and reliability of the developed hMb-RIA system.
    • To assess hMb levels in normal individuals and patients with specific medical conditions.

    Main Methods:

    • Isolation and purification of human myoglobin (hMb) from muscle tissue.
    • Development of anti-hMb antibodies and radiolabeling of hMb using the Chloramine-T method.

    Related Experiment Videos

  • Validation of the hMb-RIA system through parallel assays, hemagglutination inhibition (HI) technique, and parallelism tests.

    • Main Results:

    • The developed hMb-RIA system demonstrated high sensitivity (2 ng/ml) and low intra-assay error (2.26%).
    • Serum hMb levels were significantly higher in patients with hypothyroidism and hyperthyroidism compared to normal subjects.
    • Elevated hMb concentrations were observed in patients with acute myocardial infarction (AMI), ranging from 123 to 1510 ng/ml.

    Conclusions:

    • The developed double antibody RIA system is a sensitive and accurate method for measuring serum hMb.
    • The assay can differentiate hMb levels in normal individuals from those with thyroid dysfunction and AMI.
    • Further studies are needed to establish normal hMb reference ranges based on sex and age for comprehensive clinical application.