Nuclear chromatin changes in hepatocytes of soman treated rats

Cytochemical and morphometric indices of DNA metabolism and nuclear transcriptional-translational capabilities were used to monitor liver responses in Sprague-Dawley X Wistar rats subcutaneously injected with 0.9 LD50 soman, a highly toxic acetylcholinesterase-inhibiting organophosphate. Thirty minutes post-soman the following were evident: (1) increased polyploidization; (2) expansion of the nuclear envelope; and (3) elevated deoxyribonucleoprotein (DNP) complex lability to acid hydrolysis. Nuclear changes were accompanied by cytoplasmic correlates of impaired hepatocyte function, i.e., vacuolation and depressed basophilia. The overall data suggest that with acute soman toxication there are concomitant adaptive and maladaptive responses occurring within liver parenchymal elements. It is postulated that nuclear changes represent an early proliferative response and DNP activation to enhance detoxification capabilities, whereas cytoplasmic changes reflect the disruption of normal function and hepatotoxic injury.