[Fibroblast adhesion to glass and the dynamics of pH change in the medium in the pericellular space]

A study was made of dynamics of pH changes in the pericellular space of the Chinese hamster fibroblasts after the completion of cell sedimentation in three media. With the first medium, the cells were spread over the glass, with the second one they were attached to the glass without spreading and with the third medium the cells were not attached to the glass at all. The pH value of the medium surrounding the cells decreased, mainly, within the first 6 hours after sedimentation was completed, this depended on the cell population density on the glass surface. The outlook of this dependence was different for the three media. Based on the analysis of the results obtained it is concluded that the attachment of cells to glass may lead to the increased efflux of acid metabolic products from the cell.