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Immunomodulation by Propionibacterium granulosum KP 45 and experimental trichinellosis in mice
Abstract:
Swiss mice were infected orally with 200 Trichinella spiralis larvae and injected intraperitoneally with a potent immunomodulator (isolated cell walls of Propionibacterium granulosum strain KP 45). In control mice 22 871 +/- 9913 larvae were found in the whole body, whereas in mice treated with P. granulosum KP 45 on the day of inoculation 9450 larvae +/- 8244 larvae were counted (p less than 0.01). Immunomodulation 7 days before or 7 days after T. spiralis inoculation gave less pronounced effects but the decreasing number of larvae was still significant. It is suggested that the above effects are due to an activation of macrophages.
Insights
This study shows that Propionibacterium granulosum KP 45 immunomodulation significantly reduces Trichinella spiralis larvae in mice. This suggests a potential therapeutic strategy for parasitic infections by activating immune responses.
Area of Science:
- Immunology
- Parasitology
- Microbiology
Background:
- Trichinella spiralis is a parasitic nematode causing trichinellosis.
- Effective treatments for trichinellosis are crucial for public health.
- Immunomodulators are being explored for their therapeutic potential.
Purpose of the Study:
- To investigate the effect of Propionibacterium granulosum KP 45 on Trichinella spiralis infection in mice.
- To determine if P. granulosum KP 45 can reduce parasitic load.
- To explore the role of macrophage activation in this immunomodulatory effect.
Main Methods:
- Swiss mice were infected with Trichinella spiralis larvae.
- Mice received intraperitoneal injections of isolated cell walls of Propionibacterium granulosum strain KP 45.
- Parasitic larvae counts were performed in control and treated groups.
Main Results:
- Mice treated with P. granulosum KP 45 showed a significant reduction in T. spiralis larvae compared to controls (9450 vs 22871 larvae).
- Immunomodulation administered before or after infection also resulted in significant larvae reduction.
- The observed effects suggest macrophage activation as a potential mechanism.
Conclusions:
- Propionibacterium granulosum KP 45 exhibits significant immunomodulatory effects against Trichinella spiralis infection in mice.
- Early or delayed administration of the immunomodulator is effective in reducing parasitic load.
- Macrophage activation is proposed as the underlying mechanism for the observed therapeutic benefits.