Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium binding by lithocholic acid derivatives.

D G Oelberg, W P Dubinsky, E W Adcock

    The American Journal of Physiology
    |July 1, 1984
    PubMed
    Summary

    Cholestatic bile salts lithocholic acid (LCA), its sulfate (LCS), and glucuronide (LCG) derivatives bind calcium (Ca) 10-60 times more strongly than other bile salts. This high affinity explains their potent cholestatic effects.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Service users' experiences of receiving a diagnosis of borderline personality disorder: A systematic review.

    Personality and mental health·2020
    Same author

    Advances in the systemic treatment of triple-negative breast cancer.

    Current oncology (Toronto, Ont.)·2018
    Same author

    Reviews.

    Journal of wound care·2016
    Same author

    Vascularised muscle - an aid to bone union.

    Journal of wound care·2016
    Same author

    Sex pheromones of rice moth,Corcyra cephalonica Stainton : I. Identification of male pheromone.

    Journal of chemical ecology·2013
    Same author

    Sex pheromones of rice moth,Corcyra cephalonica Stainton : II. Identification and role of female pheromone.

    Journal of chemical ecology·2013

    Area of Science:

    • Biochemistry
    • Hepatology
    • Physiology

    Background:

    • Lithocholic acid (LCA) and its derivatives, lithocholic acid sulfate (LCS) and lithocholic acid glucuronide (LCG), are known potent cholestatic agents.
    • Precipitation of calcium (Ca) salts of LCG occurs during LCG-induced cholestasis in rats, suggesting an interaction between bile salts and Ca.

    Purpose of the Study:

    • To investigate the affinity of various bile salts, including LCA, LCS, and LCG, for calcium (Ca).
    • To correlate the binding affinity for Ca with the cholestatic potential of these bile salts.

    Main Methods:

    • Solutions of selected bile salts were titrated with Ca.
    • Apparent equilibrium constants (KcaBS) were determined by measuring unbound Ca ion concentrations.
    • Unbound Ca ion concentrations were measured using spectrophotometry with antipyrylazo III or murexide, or a Ca-selective electrode.

    Main Results:

    • Cholestatic bile salts LCS, LCG, and LCA exhibited significantly higher Ca binding affinities (KcaBS values ranging from 1.12 x 10(-4) M to 3.09 x 10(-4) M) compared to taurocholic acid (TC), glycocholic acid (GC), and taurolithocholic acid sulfate (TLCS) (KcaBS values ranging from 1.93 x 10(-3) M to 6.07 x 10(-3) M).
    • LCS, LCG, and LCA bind Ca 10-60 times more avidly than TC, GC, and TLCS.
    • At physiological unbound Ca ion concentrations (approx. 1 mM) found in serum or bile, only LCS, LCG, and LCA are expected to bind significant amounts of Ca.

    Conclusions:

    • The potent cholestatic properties of LCS, LCG, and LCA are strongly associated with their high affinity for calcium.
    • The preferential binding of Ca by these specific bile salts may contribute to their precipitation and subsequent cholestasis.

    Related Experiment Videos