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Solid-phase optoelectronic sensors for biochemical analysis.

M J Goldfinch, C R Lowe

    Analytical Biochemistry
    |May 1, 1984
    PubMed
    Summary
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    Simple optoelectronic sensors detect penicillin, urea, and glucose using enzyme-dye membranes. These reagentless sensors offer reproducible results for various analytes, enabling cost-effective monitoring.

    Area of Science:

    • Analytical Chemistry
    • Biomedical Engineering
    • Materials Science

    Background:

    • Optoelectronic sensors offer sensitive detection methods.
    • Enzyme-based biosensors are crucial for analyte quantification.
    • Solid-phase sensor development aims for simplified, reagentless operation.

    Purpose of the Study:

    • To develop simple, solid-phase optoelectronic sensors for penicillin, urea, and glucose.
    • To integrate triphenylmethane dyes with enzymes for substrate detection.
    • To create inexpensive and reagentless sensing devices.

    Main Methods:

    • Co-immobilization of derivatized triphenylmethane dyes (bromcresol green, bromthymol blue) with enzymes (penicillinase, urease, glucose oxidase) onto a transparent membrane.
    • Sandwiching the membrane between a red-light-emitting diode and a silicon photodiode with an integral amplifier.

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  • Monitoring changes in output voltage due to pH-induced color shifts in the membrane upon substrate presence.

    • Main Results:

    • The optoelectronic sensors demonstrated reproducible responses for penicillin G, urea, and D-glucose across a 0-10 mM concentration range.
    • Catalytic action of enzymes on substrates caused detectable perturbations in local pH, leading to color changes.
    • The system effectively monitored analyte concentrations via changes in detector output voltage.

    Conclusions:

    • Simple, inexpensive, and reagentless optoelectronic sensors for penicillin, urea, and glucose have been successfully constructed and operated.
    • The developed sensors provide a reproducible and efficient method for analyte detection.
    • This technology holds promise for simplified point-of-care diagnostics and environmental monitoring.