Antimicrobial susceptibility of Prototheca zopfii isolated from bovine intramammary infections
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Abstract:
In vitro antimicrobial susceptibility tests were carried out on 48 strains of Prototheca zopfii, an achlorophyllous algae causing refractory mastitis in dairy cows; 27 antimicrobials were evaluated. All strains were susceptible to both myxin and nystatin. In addition, 22 strains were susceptible to amphotericin B, 21 to polymyxin B, and 18 to gentamicin. Only 1 strain was susceptible to kanamycin. All strains were resistant to ampicillin, bacitracin, carbenicillin, cephalothin, chloramphenicol, clotrimazole, cloxacillin, erythromycin, flucytosine, ketoconazole, lincomycin, miconazole, neomycin, nitrofurazone, novobiocin, oleandomycin, penicillin, rifampin, streptomycin, tetracycline, and vancomycin.
Insights
Prototheca zopfii, a cause of dairy cow mastitis, showed susceptibility to myxin and nystatin in antimicrobial tests. Most strains were resistant to common antibiotics, highlighting limited treatment options for this algal infection.
Area of Science:
- Veterinary Mycology
- Dairy Cattle Health
- Antimicrobial Resistance
Background:
- Prototheca zopfii is an achlorophyllous alga responsible for mastitis in dairy cows.
- Refractory mastitis cases necessitate understanding antimicrobial efficacy against Prototheca species.
Purpose of the Study:
- To evaluate the in vitro antimicrobial susceptibility of 48 Prototheca zopfii strains.
- To identify effective antimicrobial agents for treating Prototheca-induced mastitis in cattle.
Main Methods:
- In vitro antimicrobial susceptibility testing was performed.
- 48 strains of Prototheca zopfii were tested against 27 different antimicrobials.
Main Results:
- All 48 strains were susceptible to myxin and nystatin.
- Significant susceptibility was observed for amphotericin B (22 strains), polymyxin B (21 strains), and gentamicin (18 strains).
- Kanamycin showed limited efficacy, with only one strain susceptible. All strains were resistant to 21 other tested antimicrobials, including common antibiotics like penicillin and tetracycline.
Conclusions:
- Myxin and nystatin are potential therapeutic agents for Prototheca zopfii mastitis.
- The widespread resistance to conventional antibiotics underscores the challenge in treating this condition.
- Further research into effective treatments for Prototheca-induced mastitis is warranted.