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Hepatic metabolite concentration in lactic acidotic sheep
American Journal of Veterinary Research
|June 1, 1984
Summary
Lactic acidotic sheep show lower liver glycogen and pyruvate, increasing the lactate-to-pyruvate ratio. Impaired carbohydrate availability, not oxygen, likely limits liver energy production in these animals.
Area of Science:
- Biochemistry
- Animal Physiology
- Metabolic Disorders
Background:
- Lactic acidosis is a critical condition affecting energy metabolism.
- Hepatic function is crucial for maintaining metabolic homeostasis.
- Understanding the mechanisms of hepatic dysfunction in lactic acidosis is vital.
Purpose of the Study:
- To investigate hepatic metabolite concentrations in lactic acidotic sheep.
- To assess hepatic oxygen consumption in normal versus acidotic sheep.
- To determine the limiting factors of aerobic glycolysis in lactic acidosis.
Main Methods:
- Measurement of hepatic metabolite concentrations (e.g., pyruvate, glycogen).
- Quantification of hepatic oxygen consumption.
- Comparison between clinically normal and lactic acidotic sheep models.
Main Results:
- Acidotic sheep exhibited significantly lower liver pyruvate concentrations (P < 0.005).
- Elevated L-lactate to pyruvate ratios were observed in acidotic sheep.
- Significantly reduced liver glycogen concentrations were found in acidotic sheep (P < 0.005).
- Hepatic oxygen consumption was depressed in acidotic sheep compared to normal sheep.
Conclusions:
- Limited carbohydrate substrate availability, not oxygenation, appears to restrict aerobic glycolysis in the liver of lactic acidotic sheep.
- Hepatic metabolic alterations are central to the pathophysiology of lactic acidosis in sheep.
- Further research into substrate-supplementation therapies may be warranted.