Ocular fluorophotometry using high S-N ratio fluorophotometer

fluorophotometer was remolded to improve S-N (Sensitivity-Noise) ratio by means of photomultiplier cooling system, and ocular fluorophotometry was performed in 13 subjects aged from 20 to 40 (mean 32.5) years old and 13 subjects aged from 55 to 62 (mean 59.2) years old to assess aging effect on the blood-ocular barrier. The results revealed that the fluorescence value at the midvitreous, lenticular autofluorescence, and the anterior chamber of older group is higher than that of the younger group (p less than 0.05). The higher value of midvitreous may be derived from increase of diffusion rate due to vitreous liqueation rather than the blood-retinal barrier abnormalities.