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Adhesion of Leptospira at a solid-liquid interface: a model.

B Kefford, K C Marshall

    Archives of Microbiology
    |May 1, 1984
    PubMed
    Summary
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    Bacterial motility influences Leptospira adhesion to surfaces. Inhibiting motility increases reversible and irreversible adhesion by reducing detachment rates, suggesting a dynamic model for bacterial surface interactions.

    Area of Science:

    • Microbiology
    • Surface Science
    • Bacterial Adhesion Dynamics

    Background:

    • Saprophytic Leptospira biflexa serovar patoc exhibit both reversible and irreversible adhesion at solid-liquid interfaces.
    • Bacterial motility is a key factor in microbial surface interactions and biofilm formation.

    Purpose of the Study:

    • To investigate the role of bacterial motility in the adhesion dynamics of Leptospira.
    • To explore the effect of motility inhibition on reversible and irreversible adhesion.
    • To propose a dynamic model for Leptospira adhesion at a solid-liquid interface.

    Main Methods:

    • Studied two strains of Leptospira biflexa serovar patoc.
    • Utilized carbonyl cyanide meta-chlorophenyl hydrazone (CCCP) to inhibit bacterial motility.

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  • Employed microscopic observations to analyze bacterial behavior and adhesion.
  • Developed a dynamic model based on attachment (ON) and detachment (OFF) phases.

    • Main Results:

    • Both reversible and irreversible adhesion were enhanced by CCCP, which inhibits motility.
    • Actively motile Leptospira were observed to detach from the substratum, indicating motility's role in detachment.
    • Inhibition of motility reduced the detachment phase, leading to increased reversible adhesion.
    • Increased reversible adhesion directly correlated with increased irreversible adhesion.

    Conclusions:

    • Bacterial motility plays a crucial role in the detachment phase of Leptospira adhesion.
    • Inhibiting motility enhances both reversible and irreversible adhesion by reducing detachment.
    • A dynamic model involving attachment and detachment rates effectively describes Leptospira adhesion.