Replication of mumps virus in murine cells

Area of Science:

• Virology
• Cell Biology
• Immunology

Background:

• Mumps virus is a significant human pathogen.
• Understanding mumps virus tropism in various cell types is crucial for research.
• Murine cell models are valuable for studying viral replication.

Purpose of the Study:

• To investigate the replication efficiency of mumps virus strains in different murine cell lines.
• To identify susceptible and non-susceptible murine cell types for mumps virus replication.
• To compare the replication kinetics of the Enders strain (EY) in various cell cultures.

Main Methods:

• Inoculation of mumps virus Enders strain (EY) into established murine lymphoid and non-lymphoid cell lines.
• Assessment of viral replication yield and growth kinetics in susceptible cell lines.
• Testing replication of EY strain in primary murine culture cells, including stimulated spleen cells.
• Evaluation of replication of seven additional mumps virus strains in EL-4, L1210, and L929 cells.

Main Results:

• Eight of 16 lymphoid and 4 of 13 non-lymphoid cell lines supported EY strain replication, with higher efficiency in lymphoid cells.
• Replication kinetics varied in low-yield cell lines, but were similar to Vero cells in high-yield lines.
• Primary murine cells, including stimulated spleen cells, were not susceptible to EY strain.
• Four strains replicated in EL-4 cells, 6 strains in L1210 cells, and none in L929 cells, with variable yields.

Conclusions:

• Murine lymphoid cell lines are generally more permissive to mumps virus replication than non-lymphoid lines.
• Primary murine cells and L929 cells are largely resistant to mumps virus replication.
• Susceptibility to mumps virus varies significantly among different murine cell lines and virus strains.