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Isolation and characterization of post gamma globulin in mouse
Biochemical and Biophysical Research Communications
|July 18, 1984
Summary
Researchers isolated a 13,000-dalton protein from mouse urine following kidney dysfunction. This protein closely resembles human Post Gamma globulin based on multiple laboratory analyses.
Area of Science:
- Nephrology
- Protein Biochemistry
- Immunology
Background:
- Tubular renal dysfunction in mice can lead to the excretion of specific proteins.
- Identifying urinary proteins can provide insights into kidney health and disease.
Purpose of the Study:
- To isolate and characterize a protein found in the urine of mice with induced tubular renal dysfunction.
- To compare the isolated protein with known human proteins for potential similarities.
Main Methods:
- Induction of tubular renal dysfunction in CBA mice.
- Isolation of a protein (approximately 13,000 daltons) from mouse urine.
- Analysis using electrophoresis, amino acid content determination, and immunochemical criteria.
Main Results:
- A protein of approximately 13,000 daltons was successfully isolated.
- Electrophoretic, amino acid, and immunochemical analyses indicated similarity to human Post Gamma globulin.
Conclusions:
- The study successfully identified and characterized a novel urinary protein in mice with induced kidney dysfunction.
- The findings suggest a potential homology between this mouse urinary protein and human Post Gamma globulin, warranting further investigation.