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[Delayed hypersensitivity reaction to alloantigens in mice]
A new scheme of induction of delayed hypersensitivity (DH) to alloantigens in mice is described. The optimal conditions for DH development are intraperitoneal injection of 1 X 10(7) cells of the allogeneic spleen to normal mice (not treated with cyclophosphamide) 5 days before the challenge with 5 X 10(6) spleen cells. s.c. The maximal response was seen 24 h after resolution. The reaction was specific, being mediated by T lymphocytes; it could not be transferred to intact recipients by allogeneic serum. However, DH was found to be transferrable by s.c. injection of 5 X 10(6) lymphocytes from sensitized donors. Such a modification of the scheme of DH induction may appear useful for studies of the animals' immunity, particularly for studies of the tolerance to allografts.
A new scheme of induction of delayed hypersensitivity (DH) to alloantigens in mice is described. The optimal conditions for DH development are intraperitoneal injection of 1 X 10(7) cells of the allogeneic spleen to normal mice (not treated with cyclophosphamide) 5 days before the challenge with 5 X 10(6) spleen cells. s.c. The maximal response was seen 24 h after resolution. The reaction was specific, being mediated by T lymphocytes; it could not be transferred to intact recipients by allogeneic serum. However, DH was found to be transferrable by s.c. injection of 5 X 10(6) lymphocytes from sensitized donors. Such a modification of the scheme of DH induction may appear useful for studies of the animals' immunity, particularly for studies of the tolerance to allografts.