Stimulation of protein synthesis in isolated mammalian mitochondria by a factor in the cytosol

Dialyzed rat liver cytosol possesses the ability to stimulate the incorporation of [35S]-methionine into mitochondrial proteins. The addition of dialyzed cytosol stimulates sevenfold the rate of protein synthesis by isolated mitochondria after 10 minutes incubation and the extent of this stimulation increases with the time. Various control experiments indicate that the effect is not artifactual nor does it appear to be brought about by GDP or GTP.