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Related Experiment Videos

Splash basin contamination in orthopaedic surgery.

R A Baird, F R Nickel, L D Thrupp

    Clinical Orthopaedics and Related Research
    |July 1, 1984
    PubMed
    Summary
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    Splash basin fluid in orthopedic surgery is often contaminated, frequently with Staphylococcus epidermidis. This highlights a potential source of surgical wound contamination, necessitating precautions with implants and instruments.

    Area of Science:

    • Microbiology
    • Orthopedic Surgery
    • Infection Control

    Background:

    • The operating room environment harbors microorganisms.
    • Splash basins are used in orthopedic procedures, but their microbial load is not well-characterized.

    Purpose of the Study:

    • To determine the microbial contamination levels of splash basin fluid in orthopedic surgery.
    • To identify common microorganisms present in contaminated splash basin fluid.

    Main Methods:

    • Fluid samples were collected from 78 orthopedic surgery splash basins post-operation.
    • Samples were subjected to microbial culture to assess bacterial growth and identify organisms.

    Main Results:

    • 74% of splash basin fluid samples showed bacterial contamination.

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  • Staphylococcus epidermidis was the most frequently isolated organism.
  • 59% of positive cultures contained multiple bacterial species, and 12% had over 100 colonies/100ml.

    • Conclusions:

    • Splash basin fluid is a common reservoir for microbial contamination during orthopedic surgery.
    • Contaminated splash basins pose a risk for surgical site infections.
    • Recommendations include avoiding placement of implants and reintroduction of instruments into the operative wound from splash basins.